Current Protocols in Cell Biology is a collection of protocols designed to meet the needs of both the beginning and the experienced investigator. The protocols are detailed guides, complete with materials lists, step-by-step procedures, annotations, illustrations, and commentary. The manual covers cell culture, subcellular fractionation to isolate and study specific organelles, various types of microscopy, labeling, cell adhesion, cell and organelle motility, signal transduction, protein trafficking, macromolecular interactions, cellular aging and death, fluorescent protein technology, and study of chromosomes and nuclei.